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CABRI:ICLC |
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Accession_number |
ICLC HTL98008
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Cell_line_name |
A2780
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Brief_description |
Species: human; Tissue: ovary; Tumor: adenocarcinoma
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Description |
Species: human; Tissue: ovary; Tumor: adenocarcinoma
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Depositor |
Obtained from ECACC
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Bibliographic_reference |
-
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Morphology |
epithelial-like, grown as monolayer
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3 - 1:6 using trypsin/EDTA; seed at 3-6x10^4 cells/cm2; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
toxicity testing, cancer genetic studies
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by isoenzymes: confirmed as human with MD, NP. Validated by HLA-DP beta: confirmed as human by PCR
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Cancer Res 1985;45:2110-2115 [PMID: 3986765
]
Cancer Res 1987;47:414 [PMID: 3539322
]
Semin Oncol 1984;11:285
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